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Figure 1 | BMC Immunology

Figure 1

From: Production of monoclonal antibodies reactive with ovine eosinophils

Figure 1

Enrichment of eosinophils from mammary lavage leukocytes. Mammary lavage leukocytes obtained 72 h post challenge with Haemonchus contortus L3 larvae. (A) Cytospin preparation of MAL cells stained with Wright's stain, showing mostly eosinophils (~80%; arrows) and lower number of lymphocytes (5–10%) and macrophages (10–15%). (B) The three populations of leukocytes in MAL were resolved on the basis of forward scatter (FSC) versus side scatter (SSC).G1, eosinophils. G2, lymphocytes. G3, macrophages. (C) Cytospin preparation showing eosinophils which were gated and collected as in (B; G1) resulting in a highly enriched population of eosinophils (about 98%).

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