Figure 3
From: Production of monoclonal antibodies reactive with ovine eosinophils
Immunocytochemical staining of peripheral blood and mammary lavage cells with granulocyte-specific mAbs. Cytospin preparations of peripheral blood and MAL leukocytes were stained with Wright's stain and mAbs 1.2, 3.252 and 4.10. (A, B) Cytospots stained with Wright's stain alone show the presence of eosinophils in peripheral blood and MAL. Positive staining of eosinophils (arrows) but not other leukocytes was seen with (C, D) mAb 1.2 and (E, F) mAb 3.252. MAb 4.10 showed intense staining (arrows) of eosinophils and neutrophils in (G) blood and (H) MAL cell preparations. Bound antibody was detected using the indirect immunoperoxidase technique, followed by weak H&E staining (Original magnification ×40).