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Table 1 Reactivity of ovine granulocyte-specific monoclonal antibodies

From: Production of monoclonal antibodies reactive with ovine eosinophils

  

Antibody reactivity§

Cell reactivity

mAb

Isotype

WB

Flow cytometry

IHC

eosinophil

neutrophil

1.2

IgG1

++1

++ (ic)

++ (cy,F)

++

-

3.252

IgG1

-

++ (su,ic)

++ (cy,F,P)

++

-

4.4

IgG1

++2

++ (ic)

++ (cy,F)

+

++

4.10

IgG1

++2

-

++ (cy,F)

+

++

  1. § Western blot (WB) analysis (1reactivity detected on whole cell extracts; 2reactivity detected on eosinophil soluble and granule lysates); Flow cytometry analysis for surface (su) and intracellular (ic) reactivity; Immunohistochemistry (IHC) performed on cytospots (cy), and frozen (F) and paraffin-embedded (P) tissue sections fixed in 95% ethanol.
  2. - no reactivity; + moderate reactivity; ++ strong reactivity