Figure 4

Cluster analysis of 680 genes regulated in expression by 3M-852A and 3M-011. Gene expression analysis was performed at 4 hr post-stimulation of flow-purified pDC with 3M-852A and 3M-011 using the Affymetrix GeneChip U133A as described in Methods. D1 and D2, designate two different donors for pDC preparation in which the pDC purity was > 99%. Global gene expression was determined using the Affymetrix U133A GeneChip. Two-way hierarchical clustering was performed as described in the Methods section, using the Unweighted Pair-Group Method with Arithmetic mean (UPGMA) and the Euclidean similarity measure. The log2 fold change values were used for the analysis. Insert bar chart shows the expression change scale with red, green, and white signifying increased, decreased, and unchanged expression, respectively. Expression changes were evaluated with respect to vehicle stimulated pDC from the same donor. Expression changes for the 680 genes are documented in Additional File 1.