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Table 1 Comparison of gene expression changes as determined by Affymetrix GeneChip analysis and real time RTPCR

From: Transcriptional networks in plasmacytoid dendritic cells stimulated with synthetic TLR 7 agonists

Gene Symbol Log2 Fold Change: Affymetrix Log2 Fold Change: Real Time RTPCR
  3M-852A 3M-011 3M-852A 3M-011
IFNA1 7.2 10.2 8.9 8.0
IFNA10 5.4 6.7 7.5 7.0
IFNA13 5.5 6.6 NA NA
IFNA14 6.7 7.9 10.2 8.2
IFNA16 6.0 5.0 NA NA
IFNA17 5.1 4.3 NA NA
IFNA2 6.8 6.0 11.0 9.3
IFNA21 7.7 6.7 9.2 8.0
IFNA4 5.9 5.1 NA NA
IFNA5 6.3 5.8 9.8 8.6
IFNA6 2.6 1.3 5.0 3.7
IFNA7 5.9 4.9 NA NA
IFNA8 2.8 2.7 8.0 7.2
IFNB1 8.0 6.2 10.4 8.6
Bcl-2 1.4 1.2 3.1 2.7
BCL2L1 4.2 3.8 5.1 5.0
CCL3 2.6 1.2 3.2 0.9
CD80 2.5 1.8 5.6 4.9
CXCL10 5.2 5.7 7.5 8.3
GIP2 2.8 3.2 2.0 4.0
MX1 1.4 1.6 2.3 2.4
MYC 4.6 2.2 7.1 5.9
MYD88 1.3 1.6 2.1 2.3
PTGS2 3.5 1.2 5.4 3.1
STAT1 1.0 1.2 1.3 1.1
TNF 2.4 1.4 3.2 2.2
  1. 1Taqman primers and probes for IFNA1 in the real time RTPCR detect both FINA1 and IFNA13. Fold change in expression were calculated with respect to un-stimulated pDC. For the Affymetrix data, values are averages of data sets for four donors for 3M-852A and two donors for 3M-011. For the real time RTPCR data, values are averages of two data sets from two donors for each compound. NA designates not analyzed.