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Figure 3 | BMC Immunology

Figure 3

From: Co-inherited mutations of Fas and caspase-10 in development of the autoimmune lymphoproliferative syndrome

Figure 3

Caspase-10 activity in 293T cells transfected with P501L-caspase-10. Analysis of 293T cells transiently transfected with the mock, WTFLAG, P501LHA, or P501LHA+WTFLAG plasmids, as indicated in the panel. a) Western blot analysis of cell transfectant lysates performed with anti-caspase-10 antibody; expression of the transfected molecules was confirmed using anti-HA and -FLAG antibodies (data not shown). The white arrow shows the pro-caspase-10; black arrows indicate the cleaved forms. b) Fluorimetric enzyme assay of caspase-10 activity evaluated in the cell transfectant lysates 24 h after transfection; data are relative to those displayed by mock-transfected cells (indicated as 100%) and are the means ± SE of data from 6 independent experiments. The asterisks mark the data significantly different from those obtained with P501LHA-transfected cells (p < 0.01, Mann Whitney test).

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