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Figure 7 | BMC Immunology

Figure 7

From: Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate xenoantigens

Figure 7

Xenoantibodies expressed in germline and somatically-mutated configurations demonstrate comparable ability to block human natural IgM and IgC xenoantibody binding to pig cells. Xenoantibodies cloned from the peripheral blood of human patients mounting active xenoantibody responses were expressed as single chain xenoantibodies. The ability of xenoantibodies with mutations (clone 33) to block human natural antibody binding to pig cells was compared with that of the antibody expressed in germline configuration (clone 122B) by flow cytometry. The antibody expressed in germline configuration was as efficient as the antibody expressed in somatically-mutated form in the ability to block xenoantibody/xenoantigen interaction.

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BMC Immunology

ISSN: 1471-2172

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