Table 2 Percent inhibition of natural antibody binding to PAEC with ScFv antibodies
ScFv Antibody Clone | Percent inhibition of IgM natural antibody binding | Percent inhibition of IgG natural antibody binding | Percent decrease in complement-mediated cytotoxicity to PAEC | Binding affinity (M) of xenoantibodies for gal saccharide |
|---|---|---|---|---|
germline d10 | 56.8 +/- 4.5 | 69.6 +/- 5.4 | 20.4 +/- 4.4 | 1.1 – 1.6 × 10-8 |
mutated d21 | 52.8 +/- 3.2 | 71.4 +/- 4.5 | 18.5 +/- 5.5 | 2.3 – 4.2 × 10-8 |