Figure 3

CD43 and TcR signals induce the transcription of the T-bet gene in CD4⁺ T cells and inhibit GATA-3 gene transcription in both CD4⁺ and CD8⁺ T cells. T cells were treated for 6 h, as indicated in Figure 1. Total RNA was extracted from CD4⁺ (panel A) or CD8⁺ T cells (panel B) and subjected to real time RT-PCR analysis for T-bet or GATA-3 mRNAs as compared to the internal control GAPDH. The graphics show the fold change in the levels of the messengers, as compared to untreated cells (base line). A representative of four independent experiments is shown. Each point is the mean of duplicates which differed by <2%.