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Figure 4 | BMC Immunology

Figure 4

From: CD43 signals induce Type One lineage commitment of human CD4+ T cells

Figure 4

CD43 induces IFNγR and IL-12R expression and cooperates with IL-12 for IFNγ transcription in CD4+ T cells. CD4+ T cells were left non-stimulated (none), or were stimulated for 24 h (A), by antibody ligation of CD3 (TcR) or CD43 followed by cross-linking with a secondary antibody or were stimulated for 12 h (B) by antibody ligation of CD3 (TcR), CD43, or the simultaneous ligation of both molecules, in the presence or absence of IL-12. A) Cells were collected and stained with FITC-labelled anti-IL-12R and PE-labelled anti-IFNγR antibodies and analyzed by flow cytometry. The graphics show the fold change in the expression levels of the receptors, as compared to untreated cells. The corresponding histograms are shown to the left of the graph. B) Total RNA was extracted and subjected to real time RT-PCR analysis for IFNγ mRNA contents, as compared to the internal control GAPDH. A representative of two (A) or three (B) experiments is shown.

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