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Table 1 Co-clustering of TcR and IFNγR in response to CD43, the TcR and/or IL-12 or IL-4 signals

From: CD43 signals induce Type One lineage commitment of human CD4+ T cells

TREATMENT

% OF CELLS WITH CO-CLUSTERED RECEPTORS (Mean ± SD)

NONE

30.6 ± 4.7

CD43

59.3 ± 4.5

IL-12

53.6 ± 4.5

CD43/IL-12

54.3 ± 1.5

IL-4

38.3 ± 5.5

CD43/IL-4

39.3 ± 3.6

TCR

54.3 ± 2.3

TCR/IL-12

60.6 ± 2.0

TCR/IL-4

29.3 ± 3.6

CD43/TCR

67.3 ± 1.5

CD43/TCR/IL-12

64.6 ± 4.2

CD43/TCR/IL-4

28.7 ± 3.0

  1. CD4+ T cells were left non-stimulated or were stimulated for 12 h by antibody ligation of the TcR, CD43, or the simultaneous ligation of both molecules, in the presence or absence of IL-12 or IL-4. Cells were stained with FITC-labelled anti-CD3 and PE-labelled anti-IFNγR antibodies and analyzed with a fluorescent microscope. For each treatment we counted 100 cells and determined the percentage of cells with co-clustered receptors over those with an even distribution of receptors. Statistical significance was evaluated by the Student T test. Differences were significant with p < 0.01 for CD43, the TcR or IL-12 signals as compared with non-stimulated cells and p < 0.05 for the joint signals of CD43 and the TcR as compared with anti-CD3 or anti-CD43. The addition of IL-12 to the antibody treated cells resulted in a significant difference only for the anti-TCR treatment (p < 0.05). IL-4 significantly inhibited the co-clustering of the IFNγR and the TcR in all the antibodies treatments (p < 0.01).