Figure 3
From: Spleen tyrosine kinase Syk is critical for sustained leukocyte adhesion during inflammation in vivo
Cell diameter changes of adherent leukocytes (mean ± SEM) during local stimulation of cremaster muscle venules with fMLP. Microphotographs of an adherent control leukocyte immediately after attachment (A, left panel) and during gradual stimulation (A, right panel) leading to flattening out of the cell with a concomitant decrease in cell diameter perpendicular to the vessel wall (white arrows). Diameters of adherent leukocytes from Syk-/- chimeric mice (gray bar, n = 318 from 4 mice) and control mice (black bar, n = 419 from 6 mice) were measured perpendicular to the vessel wall before and during superfusion with fMLP (B). In addition, a cumulative frequency distribution of measured leukocyte diameters is given for Syk-/- (gray lines) and control leukocytes (black lines) after 1 min (dashed lines) and 15 min (solid lines) fMLP superfusion (C). * in (B): significant difference (p < 0.05) between Syk-/- chimeric and control mice. * in (C): significant difference (p < 0.05) in the distribution of control cell diameters at 15 min fMLP to all other groups.