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Figure 2 | BMC Immunology

Figure 2

From: Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen

Figure 2

Western Blot Analysis of Whole Tumor Cell Lysates. (A) AGN2a cell lysate was prepared (as in Methods) and the indicated immune serum used at a dilution of 1:100. Lane 1, molecular weight marker (mw); Lane 2, naïve serum; Lane 3, serum from PC61-treated mice; Lane 4, serum from AGN2a-CD80/86 immunized mice; Lane 5, serum from AGN2a-CD80/86+PC61 (Treg-depleted)treated mice. (B) SDS-PAGE analysis showing, Lane 1, mw marker (mw) and, Lane 2, total AGN2a lysate (5 × 104 cell equivalents/well).

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BMC Immunology

ISSN: 1471-2172

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