Figure 4
From: Immune system changes during simulated planetary exploration on Devon Island, high arctic
Representative flow cytometry scatter plots demonstrated the analytical integrity of the field-processed mid-mission samples. For flow cytometry analysis, whole blood was preserved with a non-cross linking preservative. This reagent was found to inhibit cellular auto fluorescence, as well as preserve antigenic structure of both extracellular as well as intracellular (cytokine) molecules. CD14 vs. side scatter plots allowing enumeration of granulocyte, monocyte and lymphocyte subsets are presented derived from a CD45+ gate to eliminate artifactual debris.