Granulysin gene expression in antigen specific T-cells. L. innocua (A) respectively L. monocytogenes (B) OVA (C) and MOCK (D) specific T-cells were cultivated as described in Materials and methods. Samples were collected before stimulation (day 0), before restimulation (day 6) and 6 days after restimulation (day 12). PCR was performed and analysed by gel electrophoresis. Values are results from semiquantitative analysis of transcripts normalised to actin from the same cDNA. Each symbol represents one donor.