Changes in expression of CD86 on tonsil plasmacytoid DC. CD123+/HLADRhi plasmacytoid DC were gated during analysis (a). Marker M1 was set using an isotype control on unactivated cells (b). CD86 was examined after activation with anti-CD40 and IFNγ (c) anti-CD40, IFNγ and LPS (d) anti-CD40, IFNγ, LPS and Poly I:C (e). The numbers in right upper corners show MFI values for region M1. The data shown comes from one representative experiment of three performed.