Goblet cell hyperplasia in S. obvelata infection. (A) Photomicrograph of colon from BALB/c, IL-4Rα-/- and IL-4-/-. Representative pictures of colon sections are shown from both naïve (i) and pinworm infected mice at 7 (ii) and 14 days PI (iii). Sections were stained with PAS to identify goblet cells. 100× magnification. (B) Quantification of goblet cells per crypt in colon 7 and 14 days PI in BALB/c (solid bar), IL-4Rα-/- (open bar) and IL-4/IL-13-/- (gray bar). Data representative of two experiments are shown. Data are means of four mice per group ± SEM. (C) S. obvelata dependent IL-4 secretion from anti-CD3 restimulated splenocytes. BALB/c (solid bar), IL-4Rα-/- (open bar). **, P < 0.01 (significantly different from naive mice). Data representative of two experiments showing means for four mice/group ± SD.