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Figure 3 | BMC Immunology

Figure 3

From: Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity

Figure 3

Prime-boost regimens of GM-OVA suppress OVA-specific CD8+ immune responses. Mice were immunized by one DNA (-/D), one adenoviral vector (-/A), two DNA (D/D), or a DNA prime adenoviral vector boost (D/A) injection. The DNA and adenoviral vector either encoded ovalbumin (OVA) or the GM-CSF-ovalbumin fusion protein (GM-OVA). One week after a single injection or the second injection the percentage of OT-I tetramer positive CD8+ cells (A) and IFN-γ and CD107a double-positive CD8+ cells after stimulation with the OT-I peptide (B) were determined. All percentages are percent of CD8+ lymphocytes. Mean percentages with SEM of three independent experiments each with three animals per group are shown. Only statistically significant differences between immunization regimens expressing ovalbumin or GM-OVA in the one-way ANOVA test are indicated.

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BMC Immunology

ISSN: 1471-2172

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