Figure 5

Coexpression of GM-CSF and ovalbumin enhances CD8+ immune responses. Mice were immunized by the DNA prime adenoviral vector boost as described in figure legend 3 with the DNA and adenoviral vectors encoding the indicated expression cassettes (s. Fig. 1A). Representative dot blots for SIINFEKL tetramer staining (left column), CD107 and IFN-γ staining (center column) and IFN-γ and IL-2 staining (right column) of CD8+ lymphocytes obtained from individual mice immunized with GM-DP-OVA (A) and OVA (B) are shown. Panel C gives the mean and SEM for the immunization groups indicated and the parameters analysed. Only statistically significant differences between the groups are indicated. Three independent immunization experiments with three animals per group were performed for the PBS, OVA, and GM-OVA groups, while one experiment was performed with a total of 6 mice for the GM-DP-OVA and the GM^rh-OVA groups.