Figure 2

Cytokine expression profile of neutrophil-like PLB-985 cells. (A) DMSO-differentiated PLB-985 cells (day 5) were cultured in the absence ("ctrl") or presence of either 100 ng/ml LPS or 100 U/ml TNFα for the indicated times. When IP-10 was measured, the cells were instead cultured for 9 h with the above stimuli, in the presence or absence of 100 U/ml IFNγ. Culture supernatants were then harvested and their cytokine content determined in ELISA. Results are representative of at least 4 independent experiments. (B) DMSO-differentiated PLB-985 cells (day 5) were pre-treated with or without MG-132 (15 μM, 30 min, 37°C), and stimulated in the absence ("ctrl") or presence of either 30 nM fMLP, 100 ng/ml LPS or 100 U/ml TNFα for 30 min at 37°C. Total RNA was extracted and cytokine mRNA expression was analyzed in RPA. Results shown in this figure are representative of at least 4 independent experiments.