Figure 4

Overexpression of E-GFP and β-galactosidase in neutrophil-like PLB-985 cells. (A) DMSO-differentiated PLB-985 (day 5) were nucleofected with a vector encoding E-GFP or empty vector (pcDNA3.1). Cells were harvested 6 h after nucleofection and processed for FACS analysis. A minimum of 10,000 cells was analyzed per sample. Results are representative of 8 independent experiments. (B) DMSO-differentiated PLB-985 (day 5) were nucleofected (squares) or electroporated (triangles) with an E-GFP vector and cultured for the indicated times prior to propidium iodide staining and FACS analysis. Results are expressed as the proportion of GFP-expressing cells in the population that excludes PI staining. Mean ± s.e.m. of 5 independent experiments. (C) DMSO-differentiated PLB-985 (day 5) were nucleofected (squares) or electroporated (triangles) with an E-GFP vector and cultured for the indicated times prior to determination of cell survival. Mean ± s.e.m. of 5 independent experiments. (D) DMSO-differentiated PLB-985 (day 5) were nucleofected with a β-galactosidase vector and cultured for 6 h. Cells were then lysed and processed for their enzymatic activity, using 1-O-[2-nitrophenyl]-β-D-galactopyranoside as a substrate. Results are of two independent experiments.