Figure 8
The effects of ATRA on Th1 and Th2 cytokine production are primarily mediated through RAR-α with minimal involvement of liganded RXRs. IL-12p70 and IFN-γ (Panel A) and IL-4 and IL-5 (Panel B) proteins were quantitated by ELISA in supernatants of 48 h, anti-CD3,-activated PBMC treated in the absence or presence of EtOH, ATRA (10-8 M), or the RAR-α agonist, AM580 (10-8 M) and in the presence or absence of EtOH (□) or the RXR agonist (10-8 M, ■). The above data are representative of the results from two different donors examined.