In vivo effect of Eucalyptus oil on activity of peripheral blood mononuclear cells from immuno-competent rats. a, Schematic diagram of rats treatment, detailed in Methods. b, Evaluation by flow cytometry of the percentage of circulating granulocytes, monocytes and lymphocytes in untreated control (yellow solid bar) and in rats after 15 days of E O administration (red solid bars). c, d, Cytofluorimetric evaluation, by phagotest kit, of phagocytic activity of granulocytes and monocytes from untreated control (yellow solid bar) and from rats after 15 days of E O administration (red solid bars). The number of internalized bacteria has been recorded as Mean Fluorescence Intensity (MFI): mean values of 12 animals/group are reported as percent of increment of MFI at T15 respect to T0. Representative plots (d) of untreated controls and Eucaliptus oil treated animals are also shown; R1: granulocyte population, R2: monocyte population; phagocytic activity in controls maintained at 0°C, to block E. coli bacteria internalization, is also reported (grey lines). e, Cytofluorimetric analysis of expression of CD44, reported as MFI, in treated and untreated rats at the end (T15) and after 5 days from the end of E O administration (T20). f, Cytofluorimetric evaluation of percentage of CD25+ monocytes in treated and untreated rats at T15 and at T20. *P < 0.01 vs control; **P < 0.001 vs control; ***P < 0.0001 vs control; ns = P not significative.