Skip to main content
Figure 1 | BMC Immunology

Figure 1

From: Id1 induces apoptosis through inhibition of RORgammat expression

Figure 1

Alteration of E2A activity in 16610D9 cells. (A) Scheme of retroviral infection and treatment of cells used for microarray analyses. Retroviral constructs are diagrammed with key components labeled. (B) FACS analysis of 16610D9 cells infected with YFP and GFP expressing retroviruses, individually or together. The left panel shows the profile of a mixture of cells infected with either YFP or GFP expressing viruses. The right panel represents the profile of cells co-infected with both types of viruses. (C) Electrophoretic mobility shift assays of E2A DNA binding activity in indicated cells with (+) or without (-) 4-OHT treatment. Oct1 binding activities were used as controls for the amount and quality of nuclear extracts. Experimental procedures are as described [19].

Back to article page