Figure 4
From: Id1 induces apoptosis through inhibition of RORgammat expression
RORγt rescues Id1-induced apoptosis. (A) Annexin V staining. 16610D9 cells were transduced with indicated retroviruses and sorted based on GFP or YFP expression from the RORγt and Id1 retrovectors, respectively. Sorted cells were cultured for 6 days before the analysis. Cells negative for 7-AAD staining were scored for Annexin-V binding by percentage. (B) Average percentages of Annexin-V positive cells were obtained from three independent experiments. Data obtained on Day 6 are shown in dark bars and those on Day 1 are presented in gray and serve as negative controls. Error bar shows standard deviation.