Cytokine expression in lung. Tlr4-deficient and control mice were sc injected with 1/5 HD wP (|▮|), aP (|▒|), or adjuvant (| |), twice before intranasal B. pertussis infection. Three and seven days after challenge the left lung lobes were excised. RNA was extracted and analyzed by real-time PCR. The fold change in mRNA expression is relative to lung tissue of untreated C57BL/6J mice. Data are indicated as mean ± SEM (N = 6). Non-boxed P-values: compared to the adjuvant control, or (when indicated) wP-vaccinated group (same strain and day after challenge). Boxed P-values: compared to wild-type mice (same treatment and day after challenge). ANOVA followed by Bonferroni. A single representative experiment of 2 is shown.