Ex vivo cytokine production by bronchial lymph node and spleen cells. Tlr4-deficient and control mice were sc injected with 1/5 HD wP (|▮|), aP (|▒|), or adjuvant (| |), twice before intranasal B. pertussis infection. Three and seven days after challenge the bronchial lymph nodes (LN) and spleens were excised and cell suspensions were made. Bronchial LN cells were cultured with Con A for 24 hr; spleen cells were cultured with heat-killed B. pertussis for 72 hr. Culture supernatants were analyzed for cytokine content by Luminex. Data are indicated as mean ± SEM (N = 6). Non-boxed P-values: compared to the adjuvant control, or (when indicated) aP-vaccinated group (same strain and day after challenge). Boxed P-values: compared to wild-type mice (same treatment and day after challenge). ANOVA followed by Bonferroni. A single representative experiment of 2 is shown.