Figure 2
From: His-tag ELISA for the detection of humoral tumor-specific immunity
Results from capture ELISA are confirmed by Western blot analysis. (A) Representative example of a sample positive by capture ELISA analyzed by Western blot using IGFBP-2 lysate. Molecular weight (lanes 1&8), Western blot control (lanes 2&7), Immunoprecipitated IGFBP-2 lysate (lanes 3&6), and immunoprecipitated His-B lysate (lanes 4&5). (B) The same sample is also positive by Western blot using recombinant IGFBP-2 protein instead of hist-tagged protein. Molecular weight (lanes 1&8), Western blot control (lanes 2&7), recombinant human IGFBP-2 (lanes 3&6), and immunoprecipitated His-B lysate (lanes 4&5).