From: His-tag ELISA for the detection of humoral tumor-specific immunity
Parameter evaluated | Assay for antibodies to IGFBP2 | Standard | |
---|---|---|---|
 | Indirect | His-tag |  |
Limit of detection, ug/ml | 0.0005 | 0.0005 | N/A |
Intra-assay cv | 12% | 13% | <15% |
Inter-assay cv | 11% | 14% | <15% |
Accuracy | 93% | 88% | Â |
(technical specificity) | (91%) | (83%) | >90% |
(technical sensitivity) | (100%) | (100%) | Â |
Calibration curve | r2 = 0.99 | r2 = 0.99 | r2 = 0.95 |
 | cv = 7% | cv = 8% | cv <15% |
Reference range, ug/ml | 0.025+/-0.148 | 0.024+/-0.051 | N/A |