Figure 5
Changes in CD8α + and CD8α - CD11b+CD11c+ cells expressing CXCR3, IFN-γ, TNF-α, IL-12p40, and CXCR3 ligands in IL-10-/- mice after mycobacterial challenge. MLN, PP, and LP lymphocytes were isolated from IL-10-/- mice challenged with cream milk alone (control) or cream milk containing 104 CFU of live or heat-killed M. avium paratuberculosis. Changes in the mean fluorescent intensities of CXCL9, CXCL10, and CXCL11 expressed by MLN CD8α+ and CD8α- CD11b+CD11c+ cells from control mice (shaded histogram) and heat-killed (dotted outlined histogram)- or live Mycobacteria (solid outlined histogram)-challenged mice are shown in Panel A. Changes in the number (± SEM) of CXCR3-, IFN-γ-, TNF-α-, IL-12p40-, CXCL9-, CXCL10- or CXCL11-expressing CD8α+ and CD8α- CD11b+CD11c+ cells from control mice as well as live or heat-killed Mycobacteria-challenged IL-10-/- mice are shown in Panel B. Asterisks indicate statistically significant differences, i.e., p < 0.01, between control and Mycobacteria-treated groups.