Figure 6
Changes in NK and NKT cells of IL-10-/- mice challenged with Mycobacteria. MLN, PP, and LP lymphocytes were isolated from IL-10-/- mice challenged with cream milk alone (control) or cream milk containing 104 CFU of live or heat-killed M. avium paratuberculosis. Changes in the mean fluorescent intensities of IL-12p40, IFN-γ, and TNF-α expressed by LP CD3+ and CD3- NK1.1+ cells from control mice (shaded histogram) or heat-killed (dotted outlined histogram)- and live MAP (solid outlined histogram)-challenged mice are shown in Panel A. Changes in the number (± SEM) of CXCR3-, IFN-γ-, TNF-α-, IL-12p40-, CXCL9-, CXCL10- or CXCL11-expressing by CD3+ and CD3- NK1.1+ cells from control mice and live or heat-killed Mycobacteria-challenged IL-10-/- mice are shown in Panel B. Asterisks indicate statistically significant differences, i.e., p < 0.01, between control and Mycobacteria-treated groups.