Figure 6
Kinetics of intracellular Ca2+ response, detected using Indo-1, AM, in U937 cells stably transfected with the non-desensitizing mutant of FPR in response to activation by Gα i /Gα s -coupled receptor agonists. Experiments were conducted as described under "Methods". A, Control cells loaded with Indo-1 were treated with fMLFF (100 nM) and DMSO. B, Cells were sequentially treated with fMLFF (100 nM) and amthamine (500 nM); C, cells were treated with fMLFF (100 nM) and isoproterenol (100 nM); D, cells were treated with fMLFF and Forskolin (40 μM). One representative experiment out of three experiments is shown.