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Figure 5 | BMC Immunology

Figure 5

From: Mast cell activation is characterized by upregulation of a functional anaphylatoxin C5a receptor

Figure 5

C5aR upregulation is independent of SCF treatment. Precursor cell-derived murine MC cultured with IL-3 were treated or not (Ø) with ionomycin, PMA, or Ag (DNP-albumin, following a 24 h preincubation period with IgE anti-DNP) for 24 h (A) or 4 h (B). In (A), MC were stained by indirect immunofluorescence and analyzed by FACS. Filled histograms indicate staining with rat IgG1 control mAb, open histograms (solid lines) with mAb 1240 against murine C5aR, and open histograms (dotted lines) with mAb 1G4 against murine C3aR. In (B), MC were labeled with PKH26 and injected i.v. into syngeneic BALB/c mice together with C5a (10 μg) i.p.. 24 h later, peritoneal cells were harvested and labeled migratory cells identified by FACS analysis. One representative experiment of 3 (A) and mean values (± SEM) of 4 independent experiments each (B) are shown.

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