Screening of functional and positional candidate genes in families with common variable immunodeficiency

Salzer, Ulrich; Neumann, Carla; Thiel, Jens; Woellner, Cristina; Pan-Hammarström, Qiang; Lougaris, Vassilis; Hagena, Tina; Jung, Johannes; Birmelin, Jennifer; Du, Likun; Metin, Ayse; Webster, David A; Plebani, Alessandro; Moschese, Viviana; Hammarström, Lennart; Schäffer, Alejandro A; Grimbacher, Bodo

doi:10.1186/1471-2172-9-3

BMC Immunology

Table 2 Primer sequences for candidate genes.

From: Screening of functional and positional candidate genes in families with common variable immunodeficiency

Gene	Exon	Forward primer	Reverse primer	bp^§	°C^§
APRIL	1	CCTTGCTACCCCACTCTTGA	TCTCTTGCACCCCCTTGAA	402	62
	2	CTGGGAAAAGGTGCGTGAGA	TGAGGAGAGAAGGAGGGAATC	337	62
	3	AGCAGCGTGGGGATTGTAA	CTCAGGTGCTTTTTGGTTCTTT	268	62
	4	AAGTGGATGCGGCTGAGATT	GGGAAGGGAGATGTTGAAGAA	536	62
	5	CATACCAAACCCAGCAGAC	GTTTCCAACCTCCCTCCTA	335	62
	6	GCGGGTCTGAGGAGTGAAGT	AGTCTAGGGGGTGGGAATGA	415	62
BCMA	1	GTCTATCTCCCTGGCACCTCTCAC	CACTGCCTTGCTTCCTTTCTCTTT	685	55
	2	CGGGAGGCTGAGGCAGGAG	TCGAAAGGGCTGTAACGAAGTGAA	451	55
	3	TCCCGACTGCTCTGTAGGCTAACG	TTCTTTCCCCCTCCCACCTTTCTC	797	57
CCL18	1	ATCCCTGGGTGCTTCCAACTC	TGCCCAGGGAGCCATCAA	414	57
	2	CCAAGCGGTGATATCTCCCAGTTC	AGGCCTTCCCCAAATGTCTCAGA	477	60
	3a	GAGGCCCCTGCAGTGTTTTGTG	CTGGGCATAGCAGATGGGACTCTT	498	60
	3b	GCAGGGGCCACAGGATTCC	TGCCCCTTTTCATATTTCCCTACT	680	57
IL10	1	GTGCCGGGAAACCTTGATTGTGG	GGGGGAATAGGTGTTGGGGATGG	435	60
	2 and 3	GGGCATCAAAAAGACCGCATTTCA	CCCCCAACGCCTGCTCAAAGA	763	60
	4	CCACCAGCTTGTCCCCTAAGTGTG	TGGCCGGCCAGCCTAACC	404	59
	5	AGCATGAGGGAGGGGAGCCTATTT	GCGCCCGGCCTAGAACCAA	781	57
IL10RA	1	CGCTAGCGCCCCAGGACAG	GTTTGCGGGTTGGGGGTTTG	545	64
	2	ATGTGCCCACTCTGCCCCTTACG	GGGCCCTCAGGCACTCACTTCATT	377	61
	3	GGGCTGTCCCAGTTTCTCCCAATG	CCCAACCCACCCCAAGACCTCTC	542	61
	4	CAAAGTCTCGGCGGGGACACC	CCTCCTCTGCCCACCCACCAT	388	61
	5	AGGCCCACCAGCTCTCAGTGTCC	CCAGGTGCACGCGTTTTGGATT	302	60
	6	CTTGGGCCACTCACTGAATGGTT	CCCACAGCGCTTGATGAAGGTAT	404	60
	7	CCTTCCCCGGCAGCACTGG	GCAGAGGAGCAGGCATGGCTAAAA	1048	63
IL10RB	1	GCGCCTTTCAAAGCTTGCGAGGAT	GGCCGTGGCGTTTGCATCTTCTCT	442	65
	2	ACCCACGTGGCCTTTGAAGACAT	GTGGCCACGAGAATTTCCCAGAC	416	60
	3	CCAGTCAGCCTCAGGGAGAC	GGGCCCCAACATAGTCACAT	486	54
	4	ACTTCCGTGGACTAATTGTTCTGC	CTTCAGGGAGGGAAAGGTCTG	494	55
	5	AGTCCCCCAAAGTGCTGTGATTAC	GGGCGATAGATTTCTGAACTGAGC	575	57
	6	TCTGAGACGTCCCCCAAGATAAAC	TGGGCAGCCCTAACTAGAAAGGAA	359	57
	7	CCAGCCAGGAGTTCTGTGAAAA	TAGATGTGGGGCTGGCTCAGAT	382	57
IL21	1 and 2	AGTTACTCACATTCATCCAT	CTGTCTCAGCAATACTACTT	736	50
	3	GAAATAGAGGATTGGGAAAG	GGTAAGGAAGACACCAGCAG	345	50
	4 and 5	TACAAGGACTTTTTCCATTG	GACTTTGCACACTTATGAGT	510	50
IL21R	1	TCTCTGCTGAGTGACCGTAAG	TTCCTTCCCCAGCCCGCTACA	675	58
	2	CAGCAAGGGCAAAATAGTCAG	TGTCTACCGCTTCTGTTTCAT	898	57
	3	TTGGGACATTTTCAGCATAGC	ACCATTCATGGGAAGCGTGTA	863	59
	4	TGCTGCCCTAAATGAGGTAGT	AAAATTAGCTGGGTGTAGTGG	610	59
	5	AGATGGGGTTTCACCGTGTTA	TGCTCGTGCCCTTGGTCTCTG	699	55
	6	TTGCTAAGATGTCTGTAGTTA	GGGAAATGGGTCTGGGGTAGA	1067	55
	7	CCCTGTTTTTCAGACGAGATA	TTCAGAGGTGTGGACTATTAC	755	55
	8	GTGAAGAGGTGGCTGTAATAG	AGATGGGGAGGCGGAGTGGTG	768	55
	9	AGTGAACCGAGATGGCACCAC	AGGGAGGACGGCTGTTGTCAT	1270	59

IBD, sporadic CVID patients with symptoms of inflammatory bowel disease; NA, not applicable.

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ISSN: 1471-2172

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