Quantitative real-time PCR analysis of the STAT1 gene expression (A) Expression analysis of STAT1 mRNA at different developmental stages. EG, egg; LV, larva; 7D, 7 day post-hatch; 14D, 14 day post-hatch; 27D, 27 day post-hatch; 34D, 34 day post-hatch. (B) Expression of STAT1 mRNA in various tissues of the flounder. MS, muscle; LV, liver; IT, intestine; ST, stomach; KD, head kidney; SK, skin; FN, fin; SP, spleen; GI, gill; EY, eye; HR, heart. Data are averages from three replications with standard deviations.