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Figure 1 | BMC Immunology

Figure 1

From: Ex vivo recovery and activation of dysfunctional, anergic, monocyte-derived dendritic cells from patients with operable breast cancer: critical role of IFN-alpha

Figure 1

(A) Percentage of PBDCs (from patients with operable breast cancer) expressing HLA-DR, CD 86 and CD 40: Statistically significant increase in expression of HLA-DR was observed in the 3 and 4 CCM (cytokine conditioned medium) compared with 2 CCM generated DCs and medium only generated DCs (p < 0.001, ANOVA). Similarly, significant increase in expression of CD86 was observed in the 3 or 4 CCM compared with 2 CCM (cytokine conditioned medium) generated DCs or medium only generated DCs (p < 0.001, ANOVA). However, CD40 expression was increased (p < 0.01, ANOVA) in the 3 or 4 CCM generated DCs compared with medium only DCs. (B) Percentage of LNDCs (from patients with operable breast cancer) expressing HLA-DR, CD 86 and CD 40: Statistically significant increase in expression of HLA-DR, CD 86 and CD40 on LNDCs cultured with 3 and 4 CCM compared with medium only generated DCs from patients with breast cancer (p < 0.01, ANOVA). (C) Percentage of PBDCs (healthy donor) expressing HLA-DR, CD 86 and CD 40: Significant increase in expression of HLA-DR was observed in the 3 and 4 CCM (cytokine conditioned medium) compared with 2 CCM generated DCs and medium only generated DCs (p < 0.001, ANOVA). However, significant (p < 0.01, ANOVA) increase in expression of CD86 was observed in the 3 or 4 CCM (cytokine conditioned medium) compared with medium only generated DCs. Similarly, CD40 expression was significantly increased in 3 or 4 CCM generated DCs compared with medium only generated DCs. Results shown in 1 A, B and C are represented as mean ± standard deviation (error bars) are from ten different patients or healthy donors. Lineage-negative cells were selectively gated and analyzed in all conditions.

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