Figure 4

Box plot showing mannose receptors in putative antigen uptake by incubation with Cascade Blue – conjugated dextran in different in vitro conditions of PBDCs generated from 10 different patients: Percentage of uptake by cells of Cascade Blue conjugated-dextran were calculated from Lin-cells (Lin-cells were gated and analyzed). PBDCs grown in 4 CCM (GM-CSF+IL-4+TNF-α+IFN-α) or 3CCM (GM-CSF+IL-4+TNF-α) compared with medium only generated PBDCs or 2 CCM (GM-CSF+IL-4), both demonstrated significantly higher levels of dextran uptake (*** p < 0.001, ANOVA). Horizontal bars represent mean value for each category. Data shown are median (line), mean (dot), interquartile range (box) and maximum and minimum values (whiskers).