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Figure 5 | BMC Immunology

Figure 5

From: Ex vivo recovery and activation of dysfunctional, anergic, monocyte-derived dendritic cells from patients with operable breast cancer: critical role of IFN-alpha

Figure 5

(A): Differential production of IL-10p40 and IL-12: Activation/maturation of PBDCs by the 3 and 4 CCM (cytokine conditioned media) leads to significantly increased production and secretion of the pro-inflammatory cytokine IL-12 p40, compared with medium only (*** p < 0.001, ANOVA) and 2 CCM generated DCs (***p < 0.001, ANOVA). In contrast, 3 and 4 CCM generated DCs produced and secreted significantly less inhibitory cytokine IL-10 compared with 2 CCM generated PBDCs (***p < 0.001, ANOVA). Cell free supernatants were obtained on day 7 of culture. Cytokine concentrations were determined by ELISA. The results shown are from ten different patients and are represented as mean ± standard deviation. Error bars represent standard deviations. (B): Production of IL-12p40 by PBDCs from healthy donors (controls): The amounts of cytokines in the samples were measured using ELISA. The levels of IL-10 and IL-12 p40 from PBDCs generated from monocytes cultured using all 4 cytokines (4 CCM) from 10 normal healthy donors were evaluated. The mean level of IL-12 p40 was 72.95 ± 4.47, which was significantly (p < 0.01, Student t-test) lower compared with 4 CCM generated PBDCs from breast cancer patients (Mean = 262 ± 15). However, IL-10 was not detectable in the supernatants and hence not shown in this Figure. (C): Cytokine production by PBDCs generated with 4 CCM from patients with operable breast cancer following stimulation with LPS (1 μg/300μl): IL-12p70 was undetectable before and after LPS stimulation and hence not shown in this Figure. IL-6 production and secretion was significantly increased after LPS stimulation (*** p < 0.001, Student t-test). NS: Not significant.

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