Skip to main content
Figure 6 | BMC Immunology

Figure 6

From: Ex vivo recovery and activation of dysfunctional, anergic, monocyte-derived dendritic cells from patients with operable breast cancer: critical role of IFN-alpha

Figure 6

Increased blastogenesis in the MLDCR: The stimulatory capacity of DCs.(PB:peripheral blood, LN:lymph node) was estimated from the incorporation of 3H-thymidine (shown as cpm). During the last 18 h on the 5th day of in vitro culture containing T cells (5 × 104 cells/well) from a normal volunteer and irradiated DCs (2.5 × 103 cells/well) from patients with operable breast cancer (PBDCs and LNDCs). DC:T cell ratio was 1:20. LNM and PBM-derived DCs with 3 and 4 CCM milieu showed a significantly increased allogeneic stimulatory capacity compared with 2 CCM (***p < 0.001, ANOVA) and medium only generated DCs (***p < 0.001, ANOVA). Results shown (mean ± standard deviation) are from ten different patients. Error bars represent standard deviations.

Back to article page