Figure 12
Morphology of the mouse urinary bladder secondary to rBCG-GFP instillation. 1.5 × 107 CFU of GFP-expressing rBCG in 200 microliters were instilled into the bladder of anesthetized mice, as described in material and methods. Twenty-four hours after instillation, the bladder were removed and frozen for morphological analyzes. H&E sections were photographed at 200× (Figure 12A) and 400× (Figure 12B). For composite H&E pictures (Fugure 12 A), at total of 9 photos were taken at 200× and automatically merged in Adobe Photoshop® CS3 extended [89]. Note that 200 microliters instilled into the bladder of anesthetized mice does not disrupt the urothelium integrity.