Figure 6
From: High-throughput gene expression profiling of memory differentiation in primary human T cells
Evaluation of LMA/Luminex detection assay reproducibility and scalability. (A) Mean expression level for each transcript in naive or memory-phenotype CD4 peripheral blood T cells were calculated and the deviation of that data point from its corresponding mean were calculated. The fraction of data points in each of 12 bins of fold deviation values is shown, representing 1100 data points (two differentiation states × 55 transcripts × 6 replicates). (B) Cumulative Z-score for multiple replicates of memory-phenotype (purple) or naive (green) CD4 T cells measured by the method carried out using robotic automation. Values > 0 show relative increase in expression of memory-phenotype signature genes; < 0 show relative increase of naive signature genes.