Figure 2

FACS analysis of 8 wk FcεRI⁺/CD117⁺ HuMC cultured in rhSCF alone, with LPS or PGN. A) HuMC numbers following culture for 72 hr in 10 ng/ml rhSCF alone and with 100 ng/ml LPS or 10 μg/ml PGN. Results are shown as the means ± SEM of 3 separate experiments performed in duplicate; B and C) Representative dot plots of HuMC expression of FcεRI (vertical axis) and CD117 (horizontal axis) following culture for 72 hr in 100 ng/ml (B) or 10 ng/ml (C) rhSCF (left-side plots), rhSCF and 100 ng/ml LPS (middle plots), and rhSCF and 10 μg/ml PGN (right-side plots) In C, CD117^low (open arrows; insert R1 histogram) and CD117^high (solid arrows; insert R2 histogram) are labeled. Representative dot plots are shown of n = 3 separate experiments performed in duplicate.