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Figure 1 | BMC Immunology

Figure 1

From: Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice

Figure 1

Schematic diagram of IL-1β-luc reporter system used for microinjection. (A) The cHS4I-hIL-1βP-Luc transgene was constructed by inserting a 4.5-kb 5' flanking promoter region of the human IL-1β gene in front of firefly luciferase cDNA. (B) Genotyping by PCR yielded a 600-bp fragment. PCR products were run on a 1% agarose gel. Lane 1 was a DL 2,000 DNA ladder, lane 2 was the product from a wild-type CBA control, lane 3 was the product from a wild-type C57BL/6 control, lane 4 was the buffer for dissolving the genomic DNA, and lanes 5–8 were samples from cHS4I-hIL-1βP-Luc heterozygous transgenic mice. P1: forward-luc primer, P2: reverse-luc primer.

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