Table 5 Restriction enzymes cutting frequency
From: Primer sets for cloning the human repertoire of T cell Receptor Variable regions
Restriction enzyme name | Sequence cleaved | Number of Functional genes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
TRAV | TRBV | TRDV | TRGV | TRAJ | TRBJ | TRDJ | TRGJ | TRBD | TRDD | TOTAL | ||
(47) | (54) | (3) | (9) | (50) | (14) | (4) | (5) | (2) | (3) | (191) | ||
ApaLI | GTGCAC | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
AscI | GGCGCGCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
BamHI | GGATCC | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 |
BglII | AGATCT | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 |
BssHII | GCGCGC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
BstEII | GGTNACC | 6 | 6 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 16 |
ClaI | ATCGAT | 0 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 |
EagI | CGGCCG | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
EcoRI | GAATTC | 3 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 7 |
EcoRV | GATATC | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 |
HaeIII | (AG)GCGC(CT) | 4 | 3 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 10 |
HindIII | AAGCTT | 3 | 4 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 11 |
KpnI | GGTACC | 7 | 26 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 41 |
NcoI | CCATGG | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 |
NdeI | CATATG | 6 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 12 |
NheI | GCTAGC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
NotI | GCGGCCGC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
PstI | CTGCAG | 11 | 25 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 37 |
PvuI | CGATCG | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SacI | GAGCTC | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 |
SacII | CCGCGG | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SalI | GTCGAC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SmaI | CCCGGG | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 |
SpeI | ACTAGT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SphI | GCATGC | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SfiI | GGCCNNNNNGGCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
XbaI | TCTAGA | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 |
XhoI | CTCGAG | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |