Figure 5
Activation of p38 MAPK in response to S. pneumoniae is dependent on TAK1 and involves MKK3. (A) RAW264.7 cells were seeded in triplicate cultures and left overnight to settle. Fifteen min prior to stimulation, some wells received 1 μM of (5Z)-7-oxozeaenol. The cells were stimulated with S. pneumoniae (5 × 107 bacteria/ml) or anisomycin (100 ng/ml) for 30 min, and whole-cell lysates were prepared. (B) Spleen cells from C57BL/6 and MKK3-/- mice were seeded in triplicates and left overnight to settle. The cells were stimulated with S. pneumoniae (5 × 107 bacteria/ml) or anisomycin (100 ng/ml) for 30 min, and whole-cell lysates were prepared. Phosphorylation of p38 MAPK was measured by Luminex, and data is presented as means +/- SEM. Similar results were obtained in 2 independent experiments.