Expression of CD94-NKG2A mRNA on CD8+T cells. Mice underwent various treatments and were divided into naïve group, PBS-AC-injected group, OVA-AC-injected group, immunized group and ACAID group. A: CD8+T cells magnetically sorted from splenic MNC of each group were lyzed to obtain total cellular RNA using TRIzol, and RT-PCR was performed to analyze CD94 and NKG2A mRNA expression. B: histograms showed the mRNA expression (mean percentage ± SEM) relative to β-action expression (set at 100%). The results shown are representative of three independent experiments. *p < 0.05 compared with naïve mice. #p < 0.05 compared with ACAID mice.