Expression of CD94-NKG2A protein on CD8+T cells. Mice underwent various treatments and were divided into naïve group, PBS-AC-injected group, OVA-AC-injected group, immunized group and ACAID group. A: Splenic MNC were harvested from each group and stained with anti-CD3, anti-CD8, anti-CD94 and anti-NKG2A mAb, then the CD3+CD8+T population was gated and analyzed for the expression of CD94 and NKG2A protein. B: FCM histogram showed the frequencies of CD8+CD94+T and CD8+NKG2A+T cells among CD8+T cells. Results represent the mean ± SEM of three separate experiments. *p < 0.05 compared with naïve mice. #p < 0.05 compared with ACAID mice.