Figure 3

Expression of granzyme B, perforin and Foxp3 in CD8⁺CD94⁺T cells. Splenic MNC from ACAID mice, immunized mice, PBS-AC-injected mice and naïve mice were prepared and labeled with anti-CD3, anti-CD8, anti-CD94, anti-perforin, anti-granzyme B and anti-Foxp3 mAb. A: CD3⁺CD8⁺T population was gated and analyzed for the frequency of CD94⁺granzyme B⁺T cells. B: CD3⁺CD8⁺T population was gated and analyzed for the frequency of CD94⁺perforin⁺T cells. C: CD3⁺CD8⁺T population was gated and analyzed for the frequency of CD94⁺Foxp3⁺T cells. The results shown are representative of three independent experiments.