IL-10 and TGF-beta1 production and the role of TGF-beta1 in immunosuppression. CD8+CD94+T or CD8+CD94-T cells(5 × 104) from ACAID mice and immunized mice were cocultured with PEC(1 × 105) and OVA (100 μg/ml) for 72 hours. ELISA was used to detect the concentration of IL-10 (A) and TGF-beta1 (B) in the supernatants of the cultures. *p < 0.05 compared with immunized mice. For blocking experiments (C), anti-TGF-beta1 mAb(50 μg/ml) or matched isotype was added to the culture at the initiation of the cell proliferation assay, then [3H]thymidine incorporation was measured to value the suppressive activity of CD8+CD94+T cells from ACAID mice. Data are represented as mean ± SEM of triplicate samples. *p < 0.05 compared with stimulated MNC+CD8+CD94+T cell group (the second bar). #p < 0.05 compared with stimulated MNC group (the first bar).