Cytokine and chemokine production in dendritic cell-platelet co-culture. Dendritic cells (DCs) were cultured for 48 h alone (untreated), with sCD154, or with untreated or fixed platelets (PLTs) either in two 0.4-μm pore-sized filter-separated compartments or in the same compartment, with or without blocking anti-CD162 mAb or suramin. Thrombin-treated PLTs were used as positive controls for cytokine and chemokine release by activated PLTs. Supernatants were collected and TNFα (A), IL-1β (B), IL12-p70 (C), and RANTES (D) production was assessed by the Luminex® technology. Histograms represent mean values ± SD of three independent experiments, with each experiment assessed in triplicate. Asterisks indicates statistical significance between assay and untreated DCs a p < 0.05 (*) or < 0.005 (**) by Mann-Whitney U test.